. Author manuscript; available in PMC: 2012 Apr 4.

Published in final edited form as: Int J Comput Biol Drug Des. 2011 Feb 4;3(4):334–349. doi: 10.1504/IJCBDD.2010.038396

Table 1.

The effects of five amino acid substitutions on protein stability. The unit of energy change is kcal/mol.

Amino acid substitution		A111V (HBA2)	T119N (HBA2)	Q61K (HRAS)	A146T (HRAS)	A693V (ZBTB20)
Structure-based Tools	ΔΔ*G_stability*	−0.75	0.06	−4.42	−1.39	−2.69
	FoldX	−4.19	10.54	−2.74	−0.22	−0.68
	PoPMuSiC-2.0	−0.49	−0.50	−0.24	−0.38	−0.05
	Dmutant	−0.48	0.32	−0.38	−0.24	−0.34
	Eris	4.28	2.29	−2.63	−1.24	−0.72
	I-Mutant 3.0 (structure version)	0.13	−0.30	0.29	−0.79	−0.03
	Consensus	Decreased	Increased	Decreased	Decreased	Decreased
Sequence-based Tools	I-Mutant 3.0 (sequence version)	Increased	Increased	Increased	Decreased	Increased
	MuStab	Increased	Decreased	Increased	Decreased	Increased
	iPTREE-STAB	Increased	Decreased	Increased	Decreased	Decreased
	Consensus	Increased	Decreased	Increased	Decreased	Increased