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Tangeretin-cisplatin combination down-regulates p-Akt and its downstream substrates. The A2780/CP70 and 2008/C13 cells were treated with either 150 μM tangeretin or its vehicle for 24 hours and then treated for another 72 hours with cisplatin or its vehicle. The cellular proteins p-Akt, Akt, p-Bad, Bad, NFκB, p-GSK-3β and GSK-3β were determined by Western blot analysis.
