Figure 6.
Using MagLev to measure the binding of BCA to several immobilized ligands. This figure presents a series of photographs showing changes in levitation height over time that result from incubating PEGA beads functionalized with five different inhibitors of BCA in the presence of 100-μM BCA dissolved in the standard levitation buffer. We used Eqn. 21 to calculate the dissociation constants of the immobilized ligands from BCA; also shown, in parentheses, are the dissociation constants of analogous ligands in solution from BCA (see Supporting Information).