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. 2001 Apr 17;98(9):5222–5227. doi: 10.1073/pnas.081075398

Figure 2.

Figure 2

Confocal microscopy demonstrates the localization of BAEBL in micronemes. (A) Dd2/Nm schizonts were double labeled with anti-BAEBL region 2 and anti-EBA-175. Schizonts immunolabeled with anti-BAEBL region 2 were stained with Alexa 488 secondary antibody (green). Schizonts labeled with anti-EBA-175 were stained with Alexa 594 secondary antibody (red). (B) Dd2/Nm schizonts were double labeled with anti-BAEBL region 6 and anti-EBA-175. Schizonts immunolabeled with anti-BAEBL region 6 were stained with Alexa 488 secondary antibody (green). Schizonts labeled with anti-EBA-175 were stained with Alexa 594 secondary antibody (red). (C) Dd2/Nm schizonts were double labeled with anti-BAEBL region 2 and anti-RAP-1 monoclonal antibody. Schizonts immunolabeled anti-BAEBL region 2 were stained with Alexa 488 secondary antibody (green). Schizonts labeled with anti-RAP-1 were stained with tetramethyl rhodamine isothiocyanate (TRITC) secondary antibody (red). (D) Dd2/Nm schizonts were double labeled with anti-BAEBL region 6 and anti-RAP-1 monoclonal antibody. Schizonts immunolabeled with anti-BAEBL region 6 were stained with Alexa 488 secondary antibody (green). Schizonts labeled with anti-RAP-1 were stained with TRITC secondary antibody (red).