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. Author manuscript; available in PMC: 2013 Mar 15.
Published in final edited form as: Free Radic Biol Med. 2012 Jan 15;52(6):1101–1110. doi: 10.1016/j.freeradbiomed.2011.12.025

Fig. 1.

Fig. 1

In vitro autokinase activities of four cysteine-to-alanine mutants expressed as a normalized percentage of GST/(NΔ661)rJAK2 activity. Sf21 cells were infected separately with baculoviruses expressing recombinant enzyme variants. Each JAK2-immunoprecipitated sample was divided into two aliquots. One aliquot was analyzed via anti-JAK2 immunoblot. The other aliquot was treated with 10 mM DTT and then assayed for radiolabeling autokinase activity. The intensity of each of the autokinase signals was calculated as a percentage of the nonmutated GST/(NΔ661)r]AK2 autokinase signal and then normalized for the anti-JAK2 signal intensity relative to the nonmutated GST/(NA661)rJAK2. The normalized autokinase activities of nonmutated GST/ (NΔ661)rJAK2, kinase-inactive K882E, and C866A, C917A C1094A and C1105A mutants are plotted left to right (n = 3; bars indicate mean±standard error). *P from <0.001 to 0.018; #P = 0.024.