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. Author manuscript; available in PMC: 2013 Mar 15.
Published in final edited form as: Free Radic Biol Med. 2012 Jan 15;52(6):1101–1110. doi: 10.1016/j.freeradbiomed.2011.12.025

Fig. 2.

Fig. 2

In situ exokinase activities of GST/(NA661)rJAK2 mutants containing serine or alanine substitutions at four critical cysteine residues. Sf21 cells were co-infected with the inactive GST/rJAK2(K882E) substrate and GST/(NA661 )rJAK2, K882E, C866S, C917S, C1094S, C1105S, C866A, C917A C1094A or C1105A mutants (lanes 1–10, respectively). (A) Immunoprecipitated proteins were analyzed via Western immunoblot with anti-JAK2, then (B) the PVDF membranes were “stripped” and reprobed with anti-phosphotyrosine antibodies. The upper arrows indicate the 140-kDa GST/ rJAK2(K882E) substrate and the lower arrows indicate the various 84-kDa GST/ (NΔ661)rJAK2 variants.