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In vitro radiolabeling autokinase activity changes due to alanine vs serine substitutions of critical cysteines in GST/(NΔ661)rJAK2.
| Site-directed mutation (% normalized autokinase activity ±SE) | |
|---|---|
| C866A (36±11) | C866S (15±8) |
| C917A (59±11) | C917S (28±7) |
| C1094A (3±1) | C1094S (32±7) |
| C1105A (11±3) | C1105S (19±4) |
