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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Pflugers Arch. 2012 Jan 17;463(4):615–624. doi: 10.1007/s00424-012-1073-3

Figure 2. High cytosolic EGTA concentration increases CatchER transient amplitude but does not affect SR Ca2+ recovery.

Figure 2

A. SR Ca2+ transients recorded in electroporated FDB fibers and recorded in whole cell patch-clamp using 0.2 (black trace) or 20mM (red trace) EGTA in the pipette solution. B. CatchER ΔF/F – [EGTA] relationship. CatchER fluorescence at nadir of the response (b) is significantly larger in 20 than 0.2mM EGTA (p < 0.001, n = 11 in 0.2 mM EGTA; n = 13 in 20mM EGTA). C. SR Ca2+ recovery is expressed as (Fc-Fb)/(Fa-Fb), where a is the average of the basal fluorescence points, and b and c are CatchER fluorescence at nadir and 2s after repolarization starts, respectively. SR Ca2+ recovery is independent of cytosolic [EGTA] (p > 0.05).