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. Author manuscript; available in PMC: 2012 Apr 4.
Published in final edited form as: Parasitology. 2011 Aug 31;139(5):574–588. doi: 10.1017/S0031182011001387

Fig. 1.

Fig. 1

Transgenesis in Strongyloides stercoralis and S. ratti mediated by the piggyBac transposon. A. Donor plasmid containing the coding sequence of gfp linked to the Ss-era-1 3′ UTR and expressed under the promoter for the S. stercoralis cellular actin Ss-act-2. Coding sequences are flanked by the gypsy retroviral insulator sequences from Drosophila and by the piggyBac inverted tandem repeats (ITR). B. Construct for in vitro transcription of mRNA encoding the piggyBac transposase. C. DIC (left) and fluorescence images (right) of an F2 transgenic S. stercoralis L3i expressing GFP. These and similar S. ratti larvae represent the first transgene-expressing individuals of the F2 generation observed to date for these parasites.