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. Author manuscript; available in PMC: 2012 Apr 4.
Published in final edited form as: Parasitology. 2011 Aug 31;139(5):574–588. doi: 10.1017/S0031182011001387

Table 2.

Preliminary findings on heritable transposon-mediated transformation and the first sustained transgene expression in the F2 generations of Strongyloides stercoralis and S. ratti

Parasite Construct No. passage attempts Total no. GFP+ F1 L3i inoculated Host Total no. (%) parasitic females recovered No. (%) GFP+ parasitic females recovered No. (%) GFP+ F2 larvae recovered
S. stercoralis * piggyBac/gypsy Ss-act-2p::gfp 4 448 Gerbil 12 (2·67) 4 (0·89) 2 (0·44)
S. ratti * piggyBac/gypsy Ss-act-2p::gfp 3 88 Rat 8 (9·09) 1 (1·13) 3 (3·39)
*

This construct is diagrammed in Fig. 1A. Parental free living females were microinjected intragonadally with the plasmid in circular form and capped mRNA encoding the piggyBac transposase transcribed in vitro from the construct diagrammed in Fig. 1B.