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. Author manuscript; available in PMC: 2012 Aug 15.
Published in final edited form as: Biochem J. 2011 Aug 15;438(1):33–37. doi: 10.1042/BJ20110009

Figure 1. ABCB1 is induced by PTC through T2R38 in Caco-2 cells.

Figure 1

(A) Caco-2 cells were incubated with PTC (10 mM) for 9 h, and mRNA levels for intestinal transporters and T2R38 were analyzed by RT-qPCR. (B) ABCB1 protein was analyzed by immunoblotting. (C and D) Caco-2 cells were transfected with a siRNA targeting human T2R38 or negative control siRNA (100 nM). 48 h post-transfection, cells were treated with PTC for 9 h. (C) mRNA levels of T2R38 and ABCB1 were analyzed by RT-qPCR. (D) ABCB1 protein was analyzed by immunoblotting. Data are mean ± SEM, n=3 for three separate experiments. *p-value < 0.05. OATP, organic anion-transporting polypeptide; 1, control; 2, siRNA T2R38; 3, siRNA negative control.