Table 1. Hematopoietic parameters.

A. Rps6 heterozygously-deleted mice. CBC analyses of transgenic mice at 6-7 weeks of age, 5-6 weeks post deletion: mean±SEM, two-tailed Student’s t-test. The absolute reticulocyte count of heterozygously-deleted Rps6 vs. control mice is 32±2×10⁴/μl, n=9 vs. 35±3×10⁴/μl, n=9; mean±SEM, two-tailed Student’s t-test, p= 0.5. A normal absolute reticulocyte count in the deleted animals is inappropriately low given their anemia. Rps6^flox/flox;Mxcre (n=3) died within 3 weeks of deletion with p(I)p(C) prior to analysis or had severe pancytopenia. B. Rpl29 and Rpl22 heterozygous mice. CBC analyses of adult mice, mean±SEM, two-tailed Student’s t-test (compared to controls). Studies are ongoing to determine if these Rpl mutants have tissue-specific ribosome biogenesis defects.

A.
	Rps6 hets (n = 18)	Controls (n= 24)	p
WBC, k/μl	3.7 ± 0.3	9.0 ± 0.4	<4E-14
ANC, k/μl	0.9 ± 0.1	2.1 ± 0.1	<4E-10
ALC, k/μl	2.4 ± 0.2	6.2 ± 0.3	<1E-12
RBC, m/μl	5.6 ± 0.3	9.3 ± 0.1	<3E-12
HGB, g/dl	9.2 ± 0.4	13.9 ± 0.2	<2E-9
HCT, %	32.4 ± 1.4	45.2 ± 0.4	<3E-8
MCV, fL	58.4 ± 0.8	48.7 ± 0.4	<2E-11
MCH, pg	16.6 ± 0.3	15.0 ± 0.2	<5E-5
MCHC, %	28.4 ± 0.3	30.4 ± 0.5	0.005
RDW, %	27.3 ± 1.1	19.1 ± 0.5	<9E-7
Platelets, k/μl	1679.5 ± 81.9	900.5 ± 24.8	<2E-8

B.
	rpL29 hets (n = 4)	Controls (n= 4)	p	rpL22 hets (n = 4)	Controls (n= 4)	p
WBC, k/ul	11.2 ± 1.0	12.1 ± 1.0	0.52	11.4 ± 0.8	9.6 ± 1.6	0.35
ANC, k/ul	2.2 ± 0.4	2.3 ± 0.3	0.86	2.1 ± 0.2	1.6 ± 0.2	0.13
ALC, k/ul	8.7 ± 0.7	9.4 ± 1.1	0.60	8.6 ± 0.7	7.5 ± 1.4	0.50
RBC, m/ul	10.7 ± 0.1	10.3 ± 0.3	0.26	10.1 ± 0.5	10.0 ± 0.3	0.87
HGB, g/dl	15.7 ± 0.2	14.9 ± 0.6	0.22	14.5 ± 0.6	14.9 ± 0.3	0.54
HCT, %	48.3 ± 0.8	45.9 ± 2.2	0.36	45.6 ± 1.7	46.1 ± 0.9	0.78
MCV, fL	45.1 ± 0.7	44.6 ± 0.9	0.66	45.2 ± 2.3	46.0 ± 1.4	0.76
MCH, pg	14.7 ± 0.1	14.5 ± 0.2	0.42	14.3 ± 0.1	14.9 ± 0.3	0.06
MCHC, %	32.6 ± 0.2	32.5 ± 0.4	0.87	31.8 ± 1.3	32.3 ± 0.5	0.72
RDW, %	17.8 ± 0.2	17.7 ± 0.4	0.87	18.7 ± 0.6	18.3 ± 0.4	0.60
Platelets, k/ul	846.5 ± 36.0	1129.5 ± 215.5	0.24	740.5 ± 57.3	855.8 ± 75.4	0.27