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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Neuroendocrinol. 2012 May;24(5):789–797. doi: 10.1111/j.1365-2826.2011.02272.x

Figure 3.

Figure 3

17β-estradiol (E2) inhibits ATP-induced [Ca2+]i transients in wild type mice.

(a) Typical indication of equal [Ca2+]i responses to repeated ATP (10 μM) stimulation (indicated by arrow) with 10 min interval under control condition. (b) Second ATP-induced [Ca2+]i response rapidly attenuated by E2 (100 nM) in dorsal root ganglion cells. (c) E2-BSA (1 μM) inhibited the ATP-induced [Ca2+]i transient. After wash-out with experimental medium, ATP response on [Ca2+]i returned to initial (control) amplitude of stimulation. (d) The effect of E2 does not desensitize upon repeated application of ATP. (e) Effect of estrogen receptor antagonist ICI 182,780 alone and application of ATP. (f) ICI 182,780 (1 μM) blocked the E2 attenuation of ATP-induced [Ca2+]i transients.