The effect of 17β-estradiol (E2) on α,β-meATP-induced [Ca2+]i transients in Wt, ERαKO, and ERβKO mice. (a) Wt mouse as a control, (b) ERαKO mouse, E2 added for 5min didn’t inhibit α,β-meATP-induced [Ca2+]i transient in control vs. after E2 treatment. (c) In ERβKO mouse, E2 stimulation significantly attenuated the α,β-meATP-stimulated [Ca2+]i transient similar to that observed in Wt mouse. Summary data represented on the right bar graphs. Values are expressed as mean±SEM. Δ[Ca2+]i were determined by subtracting the [Ca2+]i peak levels from the basal [Ca2+]i levels. *Statistically significant difference from control, p < 0.05.