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Western blot analysis of mAbs against An. gambiae midgut lysates. Seventeen mAbs were analyzed. Shown are representative mAbs of the four different patterns observed: MG4B (IgG2a), MG24C (IgG1), MG25E (IgG2a), and MG27A (IgG3). Lanes a, midgut antigens were treated under standard SDS/PAGE reducing conditions. Lanes b, in addition to standard SDS/PAGE reducing conditions midgut antigens were treated with 20 mM periodate, which cleaves carbohydrate groups from polypeptide chains.
