Fig. 3.

The effect of ouabain on thyroid cancer cell proliferation. A, Cells were treated with ouabain at the indicated concentrations (T), and proliferation rates were tested using CyQuant proliferation assay kit. Error bars indicate mean ± sd. *, P values between 0.05 and 0.01; *, P < 0.01. Ouabain treatment inhibited thyroid cancer cell spheroid growth. B and C, Tumor spheroids from FTC-133 (B) and XTC-1 (C) cells were treated with ouabain at the indicated concentrations. The photographs were taken after 2–3 wk of treatment. Magnification, ×6.3.