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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Protein Expr Purif. 2012 Feb 2;82(2):270–278. doi: 10.1016/j.pep.2012.01.012

Figure 1.

Figure 1

Construction of the glycosylated or non-N-glycosylated soluble porcine CTLA4-6xHis (pCTLA4-6xHis) into pwPICZalpha between XhoI and EcoRI sites. pwPICZalpha was derived from the pPICZalpha vector (Invitrogen). pwPICZalpha contains a unique XhoI site and the original BamHI and NcoI sites were removed by mutation. AOX1: AOX1 region; AOX1 TT: AOX1 trascription termination region; TEF1: TEF1 promoter region; EM7: EM7 promoter region; Zeocin: Zeocin resistance gene (Sh ble ORF); CYC1: CYC1 transcription termination region; ColE1: ColE1 origin (pUC-derived).