Skip to main content
. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Protein Expr Purif. 2012 Feb 2;82(2):270–278. doi: 10.1016/j.pep.2012.01.012

Figure 2.

Figure 2

Purification for glycosylated soluble porcine CTLA-4: A) First step purification using Ni-Sepharose 6 fast flow resin. Lane 1 and 14: protein markers; Lane 2: sample; Lane 3: flowthrough; Lane 4: washing; Lane 5–12: eight 50 mL elution fractions using 100 mM imidazole; Lane 13 and 15–21: eight 50 mL elution fractions using 200 mM imidazole. B) Second step purification using strong anion-exchange resin Poros 50HQ. Lane 1 and 14: protein markers; Lane 2: sample; Lane 3: flowthrough; Lane 4–5: two 40 mL washing fractions; Lane 6–13: eight 10 mL elution fractions using 100 mM sodium borate; Lane 15–22: eight 10 mL elution fractions using 200 mM sodium borate; Lane 23-24: flushing using 1 M NaCl.