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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Protein Expr Purif. 2012 Feb 2;82(2):270–278. doi: 10.1016/j.pep.2012.01.012

Figure 6.

Figure 6

Blocking/competition analysis for the binding of hamster anti-mouse CD80 mAb (clone 16-10A1) to the porcine CD-80 expressing B-cell lymphoma cell line (LCL13271-5E4), shown in the column on the right, using non-biotinylated A) glycosylated or B) non-N-glycosylated porcine soluble CTLA-4. Human CD80-expressing leukemia cell line THP-1, shown in the column on the left, was included as positive control cell line for the hamster anti-mouse CD80 mAb clone 16-10A1. Concentrations listed represent the concentration of the non-biotinylated soluble porcine CTLA-4 competitor. The concentration of the biotinylated hamster anti-mouse CD80 mAb is 17 nM.