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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Cell Signal. 2012 Feb 18;24(6):1222–1228. doi: 10.1016/j.cellsig.2012.02.004

Fig. 3. Regulation of Rab23 on Gli1-mediated transcriptional activity in wild type and Su(Fu) null cells.

Fig. 3

The luciferase reporter activity of Gli1 was assessed as previously reported in either wild type fibroblasts (A, B) or Su(Fu) null fibroblasts (C). Fig. 3A shows that Rab23 negatively regulates Gli1-mediated transcriptional activity in a dose-dependent manner. The luciferase reporter activity of Gli1 was assessed as previously reported in wild type fibroblasts. Three ratios of Gli1: Rab23 were used (1:1; 1:2; 1:3). The dominant negative (D/N) form of Rab23, Rab23S23N, was also used in the study (B). Statistical analysis was performed between Gli1-Su(Fu)-wtRab23 cells and Gli1-Su(Fu)-Rab23S23N cells using Student’s t test, and the p value was 0.001. The difference between Gli1 and Gli1-Rab23 in Su(Fu) null cells was not statistically significant (C, p value= 0.6).