. Author manuscript; available in PMC: 2013 Apr 1.

Published in final edited form as: Mol Cell Probes. 2011 Dec 30;26(2):104–106. doi: 10.1016/j.mcp.2011.12.003

Table 2.

Limit of detection with qPCR

Cryptosporidium oocysts	Conventional DNA extraction and qPCR	Capture DNA extraction and qPCR	ELISA OD^a
10⁵	28.8 ± 0.04	17.0 ± 0.01	0.250 (+)
10⁴	32.3 ± 0.01	17.2 ± 0.12	0.067 (-)
10³	38.6 ± 0.62	18.0 ± 0.03	0.057 (-)
10²	nd	18.2 ± 0.04	0.059 (-)
10¹	nd	20.6 ± 0.02	0.065 (-)
0	nd	nd	0.067 (-)
Giardia cysts

10⁵	24.1 ± 0.04	26.4 ± 0.08	3.037 (+)^a
10⁴	27.3 ± 0.09	26.6 ± 0.04	1.698 (+)
10³	29.9 ± 0.06	26.9 ± 0.08	0.205 (+)
10²	31.7 ± 0.16	27.5 ± 0.01	0.067 (-)
10¹	nd	28.5 ± 0.02	0.063 (-)
0	nd	nd	0.059 (-)

For ProSpecT Giardia and Cryptosporidium ELISA tests, a positive result is defined as sample OD₄₅₀-negative control OD₄₅₀ ≥ 0.050. nd = not detected.