Expression analysis of ENA1 and NHA1 under cation and osmotic stress conditions. Northern blot analysis was performed with total RNA isolated from WTH99 strain and hog1 mutants grown in YPD medium containing 1 M NaCl (A), 1 M KCl (B), or 1 M sorbitol (C) at different time points (0, 10, 30 and 60 min). Each membrane was hybridized with the radioactively labeled ENA1 specific probe, washed, and developed. Subsequently the same membrane was deprobed, re-hybridized with the NHA1 specific probe, washed, and developed.