The role of Ena1 and Nha1 in cation homeostasis. (A) WT H99 strain and hog1, ena1, ena1 nha1, and nha1 mutants were grown for 16hr at 30°C in liquid YPD medium, 10-fold serially diluted and spotted on YPD (glucose-rich) or YP (glucose-starved) media containing indicated concentrations of KCl or NaCl. (B) Cells were incubated for 16hr at 30°C in liquid YPD medium, 10-fold serially diluted and spotted on YPD medium containing indicated concentrations of CaCl2 or LiCl.