The role of Ena1 and Nha1 in antifungal drug resistance. (A and C) Strains (WT H99 strain and hog1, ena1, ena1 nha1, and nha1 mutants) were spotted onto YPD medium including the indicated concentration of polyene [AMB (amphotericin B)] or azole [FCZ (fluconazole, 14 μg/ml), KCZ (ketoconazole, 0.3 μg/ml)] drugs. (B) Each C. neoformans strain was grown for 16hr at 30°C in YPD liquid medium, 10-fold serially diluted and spotted YPD (0.5 M KCl) agar containing indicated concentrations of AMB. Cells were incubated at 30°C for 72hr and photographed.