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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Protein Expr Purif. 2012 Feb 8;82(2):317–324. doi: 10.1016/j.pep.2012.01.019

Figure 3. Expression, native conformation and activity of various CBS constructs of wild type and the R266K mutant.

Figure 3

The SDS-PAGE-Western blot (A), native-PAGE-Western blot (B) and CBS activity (C) of the CBS constructs of wild type and the R266K mutant. The crude extracts (soluble fraction, 30 μg) were separated either in a 10% Tris-HCl gel using reducing denaturing conditions (A) or in a 4–15% Tris-HCl gel under native conditions (B), transferred to a polyvinylidene difluoride membrane and probed with monoclonal anti-CBS antibody (Abnova). Arrows designate the GST-CBS fusion protein, 6xHis-tagged CBS protein and native tetramers, respectively.