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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Lab Invest. 2012 Feb 13;92(4):532–542. doi: 10.1038/labinvest.2012.6

Figure 6. E2 enhances Shh signaling in endothelial cells by downregulating HIP expression.

Figure 6

(A) Shh and (B) HIP mRNA expression were evaluated in HUVECs, fibroblasts (NIH 3T3), and Schwann cells (SW10) that had been treated with 1×10−8 mol/L E2 for 6 hours; measurements were performed via qRT-PCR and normalized to endogenous 18S rRNA levels. (C) HUVECs were treated with or without E2 (6 hours) and with or without 1 μg/mL Shh (16 hours), and then Shh signaling was evaluated via the Gli-luciferase assay. ND indicates not detectable.