Table 1.

Toll like receptor gene primers and PCR conditions [18]. Positive control is 18S RNA (product size 315 bp) using 18S primer (Ambion).

Gene	Forward primer (5’---3’)	Reverse primer (5’---3’)	Product Size (bp)	Tm	Cycle
TLR1	TGCCAAATGGAACAGACAAG	GTGCCCAATATGCCTTTGTT	189	53	37
TLR2	TGATGCTGCCATTCTCATTC	CGCAGCTCTCAGATTTACCC	157	53	37
TLR3	AGGCGGGTGTTTTTGAACTA	TCTTCGCAAACAGAGTGCAT	224	53	37
TLR4	GCGTGGAGGTGGTTCCTAAT	CACCTGCAGTTCTGGGAAAC	161	57	34
TLR6	AGGGCTGGCCTGATTCTTAT	TGGCACACCATCCTGAGATA	202	55	37