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Equilibrium thermal unfolding of wildtype ubiquitin and three mutants, i, l, and g. (a) Temperature-dependent equilibrium DVE spectra of mutant i. (b) Melting curves (solid line) are constructed by thermodynamic fitting (two-state) to the SVD 2nd components (open circle). For mutant g, thermodynamic analysis is not possible due to incomplete unfolding, and a dashed line is added to assist the reader.
