Skip to main content
. Author manuscript; available in PMC: 2012 Dec 15.
Published in final edited form as: Bioorg Med Chem. 2011 Oct 18;19(24):7559–7569. doi: 10.1016/j.bmc.2011.10.027

Figure 9.

Figure 9

Western blot analysis of photolabeled products generated by probe 14 as detected with anti-HA following SPD and SDS-PAGE separation. Lane 1: Membranes lacking Rce1p (rce1Δ ste24Δ) and no UV irradiation. Lane 2: Membranes lacking Rce1p (rce1Δ ste24Δ) with UV irradiation. Lane 3: Membranes lacking Rce1p, Ste24p, and Ste14p (rce1Δ ste24Δ ste14Δ) and no UV irradiation. Lane 4: Membranes lacking Rce1p, Ste24p, and Ste14p (rce1Δ ste24Δ ste14Δ) and UV irradiation. Lane 5: Rce1p-containing membranes (RCE1-HA) and no UV irradiation. Lane 6: Rce1p-containing membranes (RCE1-HA) and UV irradiation. 14 was used at 15 μM in all instances.