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. Author manuscript; available in PMC: 2012 Apr 4.

Published in final edited form as: Gastroenterology. 2008 Jun 4;135(3):926–936.e2. doi: 10.1053/j.gastro.2008.05.079

Panel A: Hanker-Yates myeloperoxidase histochemical reaction in full thickness whole-mount of the muscularis externa displaying the recruitment of myeloperoxidase laden neutrophils into the jejunal smooth muscle syncytium of a surgically manipulated Egr-1^+/+ mouse. Panel B shows a relative decrease in the number of myeloperoxidase laden neutrophils extravasated into the muscularis of the Egr-1^−/− mouse (original magnification 250X, 24 hours after surgery). Panel C: Histogram of myeloperoxidase positive neutrophils counted in muscularis whole-mounts showing in the third bar a significant recruitment of neutrophils into whole-mounts from the 24 hour postsurgical inflamed muscularis externa of manipulated Egr-1^+/+. The fourth bar demonstrates that Egr-1^−/− mice contained significantly less neutrophils than manipulated wild-types (ANOVA with Bonferroni post-hoc comparison * = p-value < 0.05, N = 5 each).

Panel A: Hanker-Yates myeloperoxidase histochemical reaction in full thickness whole-mount of the muscularis externa displaying the recruitment of myeloperoxidase laden neutrophils into the jejunal smooth muscle syncytium of a surgically manipulated Egr-1^+/+ mouse. Panel B shows a relative decrease in the number of myeloperoxidase laden neutrophils extravasated into the muscularis of the Egr-1^−/− mouse (original magnification 250X, 24 hours after surgery). Panel C: Histogram of myeloperoxidase positive neutrophils counted in muscularis whole-mounts showing in the third bar a significant recruitment of neutrophils into whole-mounts from the 24 hour postsurgical inflamed muscularis externa of manipulated Egr-1^+/+. The fourth bar demonstrates that Egr-1^−/− mice contained significantly less neutrophils than manipulated wild-types (ANOVA with Bonferroni post-hoc comparison * = p-value < 0.05, N = 5 each).