Skip to main content
. Author manuscript; available in PMC: 2012 Apr 4.
Published in final edited form as: J Mol Biol. 2008 Aug 7;382(5):1136–1143. doi: 10.1016/j.jmb.2008.07.089

Figure 4. Lack of specific AQP4 binding of a putative AQP4 adhesion oligopeptide.

Figure 4

A. (top) Schematic of AQP4 membrane topology showing putative adhesion sequence (residues 139-142) in extracellular loop. The synthetic fluorescein-labeled 9-mer peptide (NH2-PPSVVGGLG-COOH) is shown. (bottom) Fluorescence micrographs of confluent astrocytes after incubation with indicated concentration of fluorescein-labeled 9-mer peptide for 30 min at room temperature, followed by brief fixation with 2% glutaraldehyde and washing. C. Summary of relative (background-subtracted) cell fluorescence for incubations done at indicated concentrations of the fluorescein-labeled 9-mer peptide, in the absence or presence of 50-fold excess unlabeled peptide (S.E., n=3–4). Differences not significant comparing AQP4 expressing vs. non-expressing glial and CHO cells. +/+: AQP4-expressing cells, −/− AQP4 null cells.