Fig. 2.

Effect of CFA-induced inflammation on voluntary wheel running and body weight. Each point or bar and vertical line represents the mean ± SEM of the values obtained (n=8–14 per group). (A) Time-course of the effect in the distance travelled induced by the intraplantar administration of 20 µl of CFA in both hindpaws (○), in comparison to naïve mice (●), during 1 h of free access to the activity wheel. Statistically significant differences between the baseline (time 0) and the values obtained after the administration of CFA: *p < 0.05; **p < 0.01; and between CFA-treated and naïve mice in the same day of evaluation: #p < 0.05; ##p < 0.01 (two-way repeated measures ANOVA followed by Tukey post-hoc test). (B) Percentage of distance traveled, relative to basal values, in naïve and intraplantar saline or CFA injected mice 24 h after injection. Mice were injected either unilaterally (left hindpaw) or bilaterally (both hindpaws). Statistically significant differences between the naïve group (white bar), and the different experimental groups: *p < 0.05; **p < 0.01 (one-way ANOVA followed by Tukey post-hoc test). (C) Body weight of mice after bilateral intraplantar injection of saline or CFA. There were no statistically significant differences between the values obtained in the inflamed and non-inflamed mice at any time-point tested (two-way repeated measures ANOVA followed by Tukey post-hoc test).