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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: FEMS Microbiol Rev. 2012 Mar 13;36(3):734–747. doi: 10.1111/j.1574-6976.2012.00333.x

Figure 3. Parasitophorous Vacuoles of recently internalized trypomastigotes are enriched in ceramide.

Figure 3

(A) Confocal image of an infected HeLa cell (15 min of infection) incubated with anti-ceramide (red) and anti-Lamp1 (green) antibodies. A protruding trypomastigote outlined with ceramide patches is seen on the left, while Lamp-1 enriched lysosomes – the source of acid sphingomyelinase - are observed closely associated with the parasitophorous vacuole (arrows). The DAPI-stained parasite kinetoplast (arrowhead) and the host cell and parasite nuclei are shown in blue. (B) 3D reconstruction of (A) highlighting the ceramide-enriched microdomains and the lysosomes.