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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Stem Cell Res. 2011 Dec 13;8(3):357–367. doi: 10.1016/j.scr.2011.12.004

Figure 1. Expression of APJ receptor in rat BMSCs.

Figure 1

RT-PCR, immunohistochemistry and Western blotting was applied to confirm expression of the apelin receptor APJ in rat BMSCs. a. RT-PCR detected mRNA expression of APJ in rat BMSCs. APJ primer specificity was verified by RT-PCR detection of APJ mRNA expression in human HEK293 cells as a negative control and a rat APJ-expression plasmid (pcDNA3.1+/APJ) used as a positive PCR control. b. Detection of APJ protein expression in rat BMSCs with an anti-APJ polyclonal antibody and immunoimaging. BMSCs were co-labeled with a PE-conjugated anti-rat CD90 antibody (red), and Hoechst 33342 nuclear label (blue) for total cells. Antibody reactivity was verified by detection of APJ (green) expression in stably transfected HEK293 cells (HEK293-APJ) but not in wild-type HEK293 cells. Scale bar = 50 μm. c. Western blot analysis showed APJ expression in BMSCs. The APJ level was not affected by treatment with serum deprivation (SD) or SD plus apelin-13 (5 nM). All experiments were repeated at least 3 times.