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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Stem Cell Res. 2011 Dec 13;8(3):357–367. doi: 10.1016/j.scr.2011.12.004

Figure 4. Effect of apelin-13 on cytochrome c release and caspase-3 activation.

Figure 4

Cytochrome c release and caspase-3 activation was tested 30 hrs after SD using Western blotting. a. Western blotting results of cytochrome c content in the cytosolic fraction of the cells. The cytochrome c level was high in SD treated cells. Apelin-13 showed inhibitory effect on the cytochrome c increase, especially at 5 nM concentration. b. Summary of normalized values of cytochrome c levels in the mitochondrial and cytosolic fractions of BMSCs. SD alone caused reduction of cytochrome c in the mitochondria accompanied by a corresponding increase in the cytoplasm. Apelin-13 reversed these apoptotic changes in a concentration-dependent manner. c. Caspase-3 activation was measured in cell lysates form different groups of cells. d. Quantified data of experiments in c. There was an increase in caspase-3 activity in SD treated BMSCs, Apelin-13 (1 nM) showed a significant effect of blocking this caspase-3 activation. Mean±SEM. n=3 assays per group. *P<0.05 vs SD group.