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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Alcohol Clin Exp Res. 2011 Dec 7;36(5):835–846. doi: 10.1111/j.1530-0277.2011.01673.x

Fig. 6.

Fig. 6

Effects of saturated and unsaturated diets on ileum tight junction protein and protein-adaptor mRNA levels in response to chronic alcohol feeding. USF diet significantly down-regulated mRNA levels of (A) ileum tight junction proteins and (B) protein-adaptors compared to SF diet. EtOH supplemented to the USF diet resulted in further suppression of these proteins. Intestinal tight junction protein mRNA expression was assessed by RT-PCR. The relative mRNA levels were analyzed using 2-ΔΔCt method by normalizing with 18s mRNA expression. Results are presented as fold changes over SF group set as 1. Values are mean+SEM, n=6 animals/per group, *p <0.05, one-way ANOVA with Tukey’s post hoc test.