Primary gingival keratinocytes were grown in raft cultures and treated with different concentrations of Zidovudine at either day 0 or day 8.
(Panels A, G, and M) untreated rafts; (Panels B, H and N) rafts treated with 0.5 µg/ml Zidovudine; (Panels C, I and O) rafts treated with 1 µg/ml Zidovudine; (Panels D, J and P) rafts treated with 2 µg/ml, the cMax of Zidovudine; (Panels E, K, and Q) rafts treated with 4 µg/ml Zidovudine; (Panels F, L, and R) rafts treated with 6 µg/ml Zidovudine. Rafts were harvested at the indicated time points (DPT denotes days post treatment) and stained with anti-cytokeratin involucrin. Images are at 20 X original magnification.