Primary gingival keratinocytes were grown in raft cultures and treated with different concentrations of Zidovudine at either day 0 or day 8.
((Panels A, G, M, and S) untreated rafts; (Panels B, H, N and T) rafts treated with 0.5 µg/ml Zidovudine; (Panels C, I, O, and U) rafts treated with 0.1 µg/ml Zidovudine; (Panels D, J, P, and V) rafts treated with 2 µg/ml, the cMax of Zidovudine; (Panels E, K, Q, and W) rafts treated with 4 µg/ml Zidovudine; (Panels F, L, R, and X) rafts treated with 6 µg/ml Zidovudine. Rafts were harvested at the indicated time points (DPT denotes days post treatment) and stained with anti-cytokeratin 5. Images are at 20 X original magnification. Rafts were harvested at the indicated time points (DPT denotes days post treatment) and stained with anti-cytokeratin 10. Images are at 20 X original magnification.