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. 2012 Apr 4;7(4):e33092. doi: 10.1371/journal.pone.0033092

Figure 1. Immunohistochemical staining for detection of influenza virus receptor on the surface of the trachea and larynx of tree sparrows.

Figure 1

Pair A & B: the negative controls not treated with any lectin; Pair C & D: treated with Maackia amurensis lectin which can bind to SAα2,3Gal; Pair E & F: treated with Sambucus nigra lectin which can bind to SAα2,6Gal; A, C, E: the trachea tissue sections; B, D, F: the larynx tissue sections. The tissue parts (shown with arrows) in Pair C & D rather than their counterparts in Pair A & B and Pair E & F were stained black-purple, indicating that the receptor for AIVs, SAα2,3Gal, is abundant on the surface of the trachea and larynx of tree sparrows.