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. 2012 Apr 4;7(4):e32716. doi: 10.1371/journal.pone.0032716

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Petermann et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Antibody reaction against E. coli UT5600(DE3) pSH3 (control, lane1), E. coli UT5600(DE3) displaying human CSN1S1 (lane 2) and E. coli UT5600(DE3) displaying bovine CSN1S1 (lane3) measured by SD-ELISA with a rabbit polyclonal anti- bovine CSN1S1 antibody. For detection a goat HRP conjugated anti rabbit antibody was used according to the conditions described for Fig. 3. (B) The antibody reaction against E. coli UT5600(DE3) pSH3 (control, black columns) and E. coli UT5600(DE3) displaying bovine CSN1S1 (white columns) were analyzed at different concentrations of the rabbit polyclonal anti-bovine CSN1S1 antiserum or in PBS with 3% FCS as negative control. (C) The SD ELISA against bovine CSN1S1 was repeated three times independently in triplicates at the highest antibody concentration applied (200 ng/ml) with E. coli UT5600(DE3)SH3 (control, lanes 1–3) and E. coli UT5600(DE3) displaying bovine CSN1S1 (lane4–6) in order to test the reproducibility.