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. 2012 Apr 4;7(4):e32294. doi: 10.1371/journal.pone.0032294

Figure 7. The role of Flo11 overexpression on upward growth in the plane of the Z-axis.

Figure 7

A) Microcolonies of wild-type cells (PC538) and cells overexpressing FLO11 (PC2712) were examined by microscopy at 10× after 24 h incubation at 30°C. Wild type and ste12 mats on high agar concentrations is also shown Bar = 100 microns. B) Contour mapping of z-stack rendering of the indicated microcolonies in panel 7A are shown. Bar = 30 microns. See Supplemental Movies S5 and S6. C) Wild-type (PC538), flo11Δ (PC1029), and GAL-FLO11 (PC2712) cells were spotted onto YEP-GAL medium (8%) agar atop nitrocellulose filters for 24 h at 30°C. Colonies were photographed in transmitted light. Bar = 1 cm. At right, separation of the GAL-FLO11 mat from the surface using forceps. D) Microscopic examination of the mats in panel C. Bar = 200 microns.