Table 3. Kinetic parameters of mutant enzymes for the CDNB/GSH and CuOOH/GSH reactions catalyzed by AtuGSTH1-1.

Enzyme	Km, (mM) (GSH)	Km, (mM) (CDNB)	kcat (CDNB) (% of the wild-type)
Wild-type	0.30±0.03	1.5±0.09	100
Phe22Ala	0.085±0.002	3.2±0.39	90.5
Ser25Ala	0.14±0.01	1.9±0.18	78.2
Arg34Ala	NDa	NDa	0.01
Arg187Ala	0.34±0.08	7.1±0.76	33.5

^aND: Non determined.