Table 4. The effect of viscosity on kcat for the CDNB/GSH and CuOOH/GSH reactions catalyzed by AtuGSTH1-1 and its mutants.
| Enzyme | Slope (CDNB/GSH) | Slope (CuOOH/GSH) |
| Wild-type | 0.151±0.003 | 0.339±0.008 |
| Phe22Ala | 0.189±0.003 | 0.263±0.003 |
| Ser25Ala | 0.325±0.008 | 0.318±0.005 |
| Arg187Ala | 0.140±0.005 | 0.271±0.002 |
The slopes for the wild-type and the mutant enzymes were derived from the linear plot of the relative turnover number (ko cat/kcat) as a function of relative viscosity (η/ηo) using glycerol as co-solvent.