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. 2012 Apr 4;7(4):e34827. doi: 10.1371/journal.pone.0034827

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Wuebben et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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D3 ESC infected with lentiviral constructs that express Scr, Msi2#1, Msi2#4, or Msi2#5 shRNA sequences were subcultured at 200 cells per cm². Six days later an observer unaware of sample designation scored colonies as ESC, Mixed or differentiated (Diff).