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A. RT-PCR analysis was performed to identify Nell2 isoforms. Only one ∼300 bp product corresponding to secreted Nell 2 isoform was detected (lane 1). Lane 2 is a 100 bp DNA ladder. B. Locations of the primers in the gene used for the PCR are indicated by arrows. The expected sizes of the products corresponding to secreted and non-secreted Nell2 isoforms are 308 bp and 179 bp, respectively.
