Figure 6. Electroporation-mediated in vivo transfection of RGCs with Nell2 expression construct.

A. Co-localization of pNell2-EGFP-transfected cells with FG-labeled RGCs. A few EGFP-positive cells were present in temporal retinas. Most transfected cells were in the nasal retina. Most transfected cells in the retina were colocalized with RGCs. No EGFP staining was observed in non-transfected retinas. B. Quantitative analysis of ELP-mediated transfection efficiency. Approximately 49% of RGCs in the upper nasal region of the retina were transfected with pNell2-EGFP, whereas the average number of transfected RGCs across the entire retina was approximately 13% (n = 5). *p<0.05.