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. 2012 Apr 4;7(4):e34810. doi: 10.1371/journal.pone.0034810

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Munemasa et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The cell protective effect of Nell2 was correlated with the transfection efficiency. Nell2-mediated cell protective effect for the entire retina (approximately 13% of transfected RGCs) was calculated to be only about 10%. In the nasal region of the retina with highest numbers of transfected RGCs (∼49%), the Nell2 overexpession supported survival of approximately 58% more RGCs than in non-transfected (n = 5) or pEGFP-transfected (n = 5) control retinas. *p<0.01.