Table 2. Amplicon sizes and parameters derived from RT-qPCR data analysis.

Organism	Genes	Amplicon size (bp)	Amplicon Tm (°C)	Median Cq	NTC* (Cq)	Amplification efficiency	R2	Slope	y interception
Lyngbya aestuarii CCY 9616	rrn16Sa.b	191/192	86.50	21.035	31.12	101.3	0.972	−3.292	17.859
	ppc	90	82.00	30.93	43.51**	100.4	0.968	−3.313	29.319
	purC	112	80.00	32.72	35.30**	103.4	0.921	−3.243	29.339
	rnpA	133	81.50	30.895	35.34**	105.3	0.990	−3.201	28.23
	rnpB	189	86.00	32.79	N.d.	101.3	0.987	−3.291	29.104
	secA	131	83.00	31.92	53.68**	98.6	0.912	−3.355	26.701
Nostoc sp. PCC 7120	rrn16Sa.b.c.d	191	83.50	17.865	31.83	105.4	0.981	−3.198	16.516
	ilvD	77	79.50	29.21	N.d.	99.7	0.978	−3.329	25.359
	petB	157	82.50	30.235	N.d.	94.3	0.988	−3.466	30.284
	rnpA	204	82.50	33.37	37.69**	97.5	0.980	−3.383	30.194
	rnpB	131	84.00	23.9	N.d.	100.3	0.994	−3.314	20.060
	secA	131	81.50	30.785	N.d.	99.5	0.988	−3.333	28.202
Synechocystis sp. PCC 6803	rrn16Sa.b	190	86.00	18.425	28.84	100.4	0.981	−3.312	14.654
	ppc	108	83.50	34.4358	47.57**	95.9	0.960	−3.425	30.680
	petB	179	84.50	29.63	N.d.	101.5	0.958	−3.287	28.052
	rnpB	136	81.50	26.38	42.43**	103.8	0.982	−3.233	22.366
	rpoA	198	84.00	32.875	41.41**	117.4	0.991	−2.965	28.836
	secA	113	82.00	35.04	N.d.	102.1	0.958	−3.271	31.765

^*No template control.

^**Non-specific amplicons (unstable at the specific amplicon melting temperature).

N.d. – Not detected.