Table 4. Primer nucleotide sequences and annealing temperatures (Ta) used in RT-qPCR.
Organism | Genes | Primer name | Primer Sequence (5′→3′) | Ta (°C) |
Lyngbya aestuarii CCY 9616 | rrn16Sa.b | BD16SF1 | CACACTGGGACTGAGACAC | 56 |
BD16SR1 | CTGCTGGCACGGAGTTAG | |||
ppc | ppcF | TATCGCCAAGAACCCTATCG | 56 | |
LppcR | GCTATTGTAAAGTCGCCAG | |||
purC | purCF | GATACCTGTCGTTTGTGG | 56 | |
LpurCR | GAACTTGTTGATAAGCCG | |||
rnpA | rnpAF1 | AACGTCAAATTCGAGCCG | 56 | |
rnpAR1 | AACAACTGCTCTAATTCTTGC | |||
rnpB | LrnpBF | ATCATCTGTTGTTGGTAAGG | 51 | |
LrnpBR | GAGGGCAATTATCTATCTGG | |||
secA | secAF | AACCTACTACTACGACATCC | 51 | |
secAR | ACTTAATCACCTGTTCTTTCAA | |||
Nostoc sp. PCC 7120 | rrn16Sa.b.c.d | BD16SF1 | CACACTGGGACTGAGACAC | 51 |
BD16SR1 | CTGCTGGCACGGAGTTAG | |||
ilvD | ilvDF | GGCTGTGATAAGAATATGC | 56 | |
ilvDR | CCACCGTAAACAAAGATAG | |||
petB | petBF1 | AGTTTGCCACTGGATTCG | 56 | |
petBR1 | AGAATCATCATCAACACCATC | |||
rnpA | NrnpAF | TACGCTCATTGGTGTCTCG | 56 | |
rnpAR1 | AACAACTGCTCTAATTCTTGC | |||
rnpB | NrnpBF2 | TAGGGAGAGAGTAGGCGTTG | 56 | |
NrnpBR2 | TTCTGTGGCACTATCCTCAC | |||
secA | secAF | AACCTACTACTACGACATCC | 56 | |
secAR | ACTTAATCACCTGTTCTTTCAA | |||
Synechocystis sp. PCC 6803 | rrn16Sa.b | BD16SF1 | CACACTGGGACTGAGACAC | 51 |
BD16SR1 | CTGCTGGCACGGAGTTAG | |||
ppc | ppcF | TATCGCCAAGAACCCTATCG | 56 | |
SppcR | CATCGTTTGCCGTTCTTCTG | |||
petB | SpetB1F | CCTTCGCCTCTGTCCAATAC | 56 | |
SpetB1R | TAGCATTACACCCACAACCC | |||
rnpB | rnpBF1 | CGTTAGGATAGTGCCACAG | 56 | |
rnpBR1 | CGCTCTTACCGCACCTTTG | |||
rpoA | SrpoAF | TTGGACTAATGGCAGTATTCA | 56 | |
rpoAR1 | CGCTTGAGACAGTTATAGGC | |||
secA | secAF | AACCTACTACTACGACATCC | 51 | |
SsecAR | TTAAATCCAAACCTTCCAG |