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. 2012 Apr 4;7(4):e34983. doi: 10.1371/journal.pone.0034983

Table 4. Primer nucleotide sequences and annealing temperatures (Ta) used in RT-qPCR.

Organism Genes Primer name Primer Sequence (5′→3′) Ta (°C)
Lyngbya aestuarii CCY 9616 rrn16Sa.b BD16SF1 CACACTGGGACTGAGACAC 56
BD16SR1 CTGCTGGCACGGAGTTAG
ppc ppcF TATCGCCAAGAACCCTATCG 56
LppcR GCTATTGTAAAGTCGCCAG
purC purCF GATACCTGTCGTTTGTGG 56
LpurCR GAACTTGTTGATAAGCCG
rnpA rnpAF1 AACGTCAAATTCGAGCCG 56
rnpAR1 AACAACTGCTCTAATTCTTGC
rnpB LrnpBF ATCATCTGTTGTTGGTAAGG 51
LrnpBR GAGGGCAATTATCTATCTGG
secA secAF AACCTACTACTACGACATCC 51
secAR ACTTAATCACCTGTTCTTTCAA
Nostoc sp. PCC 7120 rrn16Sa.b.c.d BD16SF1 CACACTGGGACTGAGACAC 51
BD16SR1 CTGCTGGCACGGAGTTAG
ilvD ilvDF GGCTGTGATAAGAATATGC 56
ilvDR CCACCGTAAACAAAGATAG
petB petBF1 AGTTTGCCACTGGATTCG 56
petBR1 AGAATCATCATCAACACCATC
rnpA NrnpAF TACGCTCATTGGTGTCTCG 56
rnpAR1 AACAACTGCTCTAATTCTTGC
rnpB NrnpBF2 TAGGGAGAGAGTAGGCGTTG 56
NrnpBR2 TTCTGTGGCACTATCCTCAC
secA secAF AACCTACTACTACGACATCC 56
secAR ACTTAATCACCTGTTCTTTCAA
Synechocystis sp. PCC 6803 rrn16Sa.b BD16SF1 CACACTGGGACTGAGACAC 51
BD16SR1 CTGCTGGCACGGAGTTAG
ppc ppcF TATCGCCAAGAACCCTATCG 56
SppcR CATCGTTTGCCGTTCTTCTG
petB SpetB1F CCTTCGCCTCTGTCCAATAC 56
SpetB1R TAGCATTACACCCACAACCC
rnpB rnpBF1 CGTTAGGATAGTGCCACAG 56
rnpBR1 CGCTCTTACCGCACCTTTG
rpoA SrpoAF TTGGACTAATGGCAGTATTCA 56
rpoAR1 CGCTTGAGACAGTTATAGGC
secA secAF AACCTACTACTACGACATCC 51
SsecAR TTAAATCCAAACCTTCCAG