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Unstimulated macrophages generated from human peripheral blood monocytes secrete IL-8, CCL2, and CCL5 (A) and do not secrete detectable amounts of IL-6 or MIP-1α. dU937 macrophages secrete increased levels of IL-8, CCL2, CCL20, CCL5, VEGF, and CXCL12 when compared with both control medium and undifferentiated U937 CM (B). All samples were analyzed in triplicate. *p<0.05.
